l-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of theCAN 1Gene
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چکیده
منابع مشابه
evaluation of spirulina platensis resistance to different antibiotics to find a selectable marker for genetic transformation
background spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. in the other hand, spirulina is a useful source of healthy components. it seems that the spirulina is transformable so that the introduction of a selectable marker is needed. objectives the purpose of this study was to determine a suitable selection marker for spirulina platens...
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A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrati...
متن کاملPosition-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker.
P element mediated germline transformation is an essential part of the analysis of gene function in Drosophila melanogaster. This method allows for efficient introduction of single copies of genes into random positions within the genome (1). The generation of multiple transformed lines is required, as some genes display variable expression patterns depending on the insertion site (2, 3). Elimin...
متن کاملThe application of the yeast N-acetyltransferase MPR1 gene and the proline analogue L-azetidine-2-carboxylic acid as a selectable marker system for plant transformation
The yeast N-acetyltransferase MPR1 gene has previously been shown to confer resistance to the toxic proline analogue azetidine-2-carboxylic acid (A2C) in yeast and transgenic tobacco. Here experiments were carried out to determine if MPR1 and A2C can work as a selectable marker system for plant transformation. The MPR1 gene was inserted into a binary vector under the control of the cauliflower ...
متن کاملTransformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker.
Most strains of Helicobacter pylori are naturally competent for uptake of chromosomal DNA. Transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. Transformation of a metronidazole resistance marker (MtrR) was demonstrated when either a laboratory-derived mutant or a MtrR...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369
DOI: 10.1080/00021369.1989.10869329